Caldicellulosiruptor kronotskyensis 2002

Gram-positiveBacilliMotileAnaerobic

Kingdom

Bacillati

Phylum

Bacillota

Class

Clostridia

Order

Caldicellulosiruptorales

Family

Caldicellulosiruptoraceae

Genus

Caldicellulosiruptor

Description

Cellulolytic capability is widely distributed across the domain bacteria and extremely thermophilic cellulose-degrading microorganisms are of particular fundamental and biotechnological interest owing to the presence of highly thermostable enzymes. Caldicellulosiruptor kronotskyensis (strain DSM 18902 / VKM B-2412 / 2002 is a strictly anaerobic, cellulolytic, non-spore-forming, extremely thermophilic, Gram-positive bacterium isolated from a terrestrial neutral geothermal spring in Geyser Valley in the Kronotsky National Park, region of Kamchatka (Far East, Russia). Cells are short, straight rods (3-3.2 x 0.5-0.7 mm) with flagella. Temperature range for growth is between 45-82 degrees Celsius, with optimum growth at 70 degrees Celsius. The pH range for growth is between 6.0-8.0, with optimum growth at pH 7.0. It is a chemo-organoheterotroph which is capable of fermenting a wide spectrum of carbohydrates (cellulose, starch, xylan, dextran, pectin, cellobiose, glucose, fructose, sucrose, xylose, maltose, galactose, lactose, arabinose, mannitol and yeast extract). It does not grow with pyruvate, chitin, raffinose, trehalose, ribose or peptone. (Adapted from PMID: 21216991 and 18523201). (EBI Integr8)

Taxonomy

KingdomBacillati
PhylumBacillota
ClassClostridia
OrderCaldicellulosiruptorales
FamilyCaldicellulosiruptoraceae
GenusCaldicellulosiruptor
SpeciesCaldicellulosiruptor kronotskyensis
Strain2002

Profile

Physiology
Gram staining propertiesPositive
ShapeBacilli
MobilityYes
Flagellar presenceYes
Number of membranes1
Image of Caldicellulosiruptor kronotskyensis 2002
Ecology, Host, and Life Cycle
Oxygen requirementsAnaerobic
Optimal temperature45
Temperature rangeThermophilic
HabitatHot spring
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementPairs- Singles
SporulationNonsporulating
Energy sourceheterotroph; organotroph; chemotroph
PathogenicityNo

Genome Summary

Caldicellulosiruptor kronotskyensis 2002

Accession NumberNC_014720.1

Gene Summary

Adenine Count

926791 bp

Thymine Count

918943 bp

Guanine Count

500174 bp

Cytosine Count

497877 bp

Genome Length

2843785 bp

Protein-coding Genes

2583 genes

Non-Coding Genes

60 genes

# of Chromosomes/Plasmids

1

Genes

NameLocus TagUniProtStrandCoordinatesMolecular Weight
chromosomal replication initiator protein dnaaCALKRO_RS00005A0Q3U6+83 - 144752268.9
dna polymerase iii subunit betaCALKRO_RS00015P52023+1667 - 277341384.7
dna replication/repair protein recfCALKRO_RS00020A6LPB4+2799 - 386041332.3
extracellular matrix/biofilm biosynthesis regulator rema family proteinCALKRO_RS00025Not Available+3889 - 417310818.1
dna topoisomerase (atp-hydrolyzing) subunit bCALKRO_RS00030O50627+4215 - 612271817.5
hypothetical proteinCALKRO_RS13650Not Available+6140 - 63167128.72
type ii toxin-antitoxin system death-on-curing family toxinCALKRO_RS00035Not Available+6313 - 669314258.5
dna gyrase subunit aCALKRO_RS00040P94605+6722 - 915191688.2
beta-propeller domain-containing proteinCALKRO_RS00045Q58949+9441 - 1136073855.7
atp-binding proteinCALKRO_RS14115Not Available+11613 - 117354395.29

Displaying genes 1 – 10 of 2643 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolites

128 records
Metabolite IDMetabolite nameStructureCAS number
BASm0001111keto-D-tagaturonateC6H9O7Chemical structure of keto-D-tagaturonateNot available
Average193.132Da
Monoisotopic193.0353762Da
BASm0001279(6S)-5-methyl-5,6,7,8-tetrahydrofolateC20H23N7O6Chemical structure of (6S)-5-methyl-5,6,7,8-tetrahydrofolateNot available
Average457.4399Da
Monoisotopic457.1709815Da
BASm0001358lactateC3H5O3Chemical structure of lactateNot available
Average89.071Da
Monoisotopic89.0244176Da
BASm0001650N-acetyl-9-O-acetylneuraminateC13H20NO10Chemical structure of N-acetyl-9-O-acetylneuraminateNot available
Average350.301Da
Monoisotopic350.109269428Da
BASm0001717fumarateC4H2O4Chemical structure of fumarateNot available
Average114.0563Da
Monoisotopic113.9953086Da
BASm0001779orotateC5H3N2O4Chemical structure of orotateNot available
Average155.09Da
Monoisotopic155.0098302Da
BASm0001785(2R,3R)-tartrateC4H6O6Chemical structure of (2R,3R)-tartrate87-69-4
Average150.0868Da
Monoisotopic150.0164379Da
BASm0001789(3R)-citramalateC5H6O5Chemical structure of (3R)-citramalateNot available
Average146.099Da
Monoisotopic146.0226205Da
BASm0001865diphosphateHO7P2Chemical structure of diphosphateNot available
Average174.95Da
Monoisotopic174.9213971Da
BASm0001921(S)-3-methyl-2-oxopentanoateC6H9O3Chemical structure of (S)-3-methyl-2-oxopentanoate1460-34-0
Average129.1339Da
Monoisotopic129.0551692Da

Displaying 11–20 of 128 metabolites