Rhodococcus opacus B4

Gram-positiveCocciNon-motileAerobe

Kingdom

Bacillati

Phylum

Actinomycetota

Class

Actinomycetes

Order

Mycobacteriales

Family

Nocardiaceae

Genus

Rhodococcus

Description

Rhodococci are aerobic, Gram positive actinomycetes of high G+C content, capable of morphological differentiation in response to their environment (e.g., cocci or filaments). These widely occurring organisms are of considerable environmental and biotechnological importance due to their broad metabolic diversity and array of unique enzymatic capabilities. These are of interest to the pharmaceutical, environmental, chemical and energy sectors. Specific applications include the desulphurization of fossil fuels and the industrial production of acrylamide. Rhodococci are well suited for bioremediation due to their capacity for long term survival in soil, their exceptional ability to degrade hydrophobic pollutants even in the presence of more readily assimilable carbon sources, and their ability to accumulate high levels of heavy metals.Strain B4 was isolated from a soil sample taken from the roadside in Hiroshima, Japan. It is a benzene-tolerant, Gram-postitive, non-motile, strictly aerobic rod, able to use many organic compound as its sole source of carbon and energy (adapted from PubMed 16233805). (HAMAP: RHOOB)

Taxonomy

KingdomBacillati
PhylumActinomycetota
ClassActinomycetes
OrderMycobacteriales
FamilyNocardiaceae
GenusRhodococcus
SpeciesRhodococcus opacus
StrainB4

Profile

Physiology
Gram staining propertiesPositive
ShapeCocci
MobilityNo
Flagellar presenceYes
Number of membranes1
Image of Rhodococcus opacus B4
AI-generated image based on bacteria physiology
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperatureNot Available
Temperature rangeMesophilic
HabitatNot Available
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementFilaments
SporulationNonsporulating
Energy sourceNot Available
PathogenicityNo

Gene Summary

Adenine Count

1267300 bp

Thymine Count

1273329 bp

Guanine Count

2693714 bp

Cytosine Count

2679107 bp

Genome Length

7913450 bp

Protein-coding Genes

7256 genes

Non-Coding Genes

64 genes

# of Chromosomes/Plasmids

6

Genes

NameLocus TagUniProtStrandCoordinatesMolecular Weight
phou domain-containing proteinROP_RS44995Not Available+640 - 8467167.4
clp protease n-terminal domain-containing proteinROP_RS00005Not Available+899 - 130314671.6
type ii toxin-antitoxin system hicb family antitoxinROP_RS00010Not Available-1496 - 186113004.2
nucleotidyltransferase domain-containing proteinROP_RS00020Not Available+2534 - 316022943.2
hypothetical proteinROP_RS00025Not Available+3160 - 356714561.2
hypothetical proteinROP_RS44750Not Available+3981 - 41064488.7
mfs transporterROP_RS00030Not Available-5167 - 643543695.3
fad-dependent oxidoreductaseROP_RS00035Not Available-6440 - 779847449.0
metalloregulator arsr/smtb family transcription factorROP_RS00040Not Available+7901 - 877031248.1
merr family transcriptional regulatorROP_RS00045Not Available+8841 - 961427778.2

Displaying genes 1 – 10 of 8246 in total

Metabolites

145 records
Metabolite IDMetabolite nameStructureCAS number
BASm0000237(R)-4'-phosphopantothenateC9H18NO8PChemical structure of (R)-4'-phosphopantothenateNot available
Average299.2149Da
Monoisotopic299.0770031Da
BASm0000249(2E,4E)-2,4-dichloromuconateC6H2Cl2O4Chemical structure of (2E,4E)-2,4-dichloromuconateNot available
Average208.98Da
Monoisotopic207.9341111Da
BASm00002512-dehydropantoateC6H9O4Chemical structure of 2-dehydropantoateNot available
Average145.1333Da
Monoisotopic145.050083776Da
BASm0000377(S)-malateC4H4O5Chemical structure of (S)-malateNot available
Average132.0716Da
Monoisotopic132.005873238Da
BASm0000387(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6Chemical structure of (6R)-5,10-methylene-5,6,7,8-tetrahydrofolateNot available
Average455.432Da
Monoisotopic455.1564286Da
BASm0000401(S)-2-succinylamino-6-oxoheptanedioateC11H12NO8Chemical structure of (S)-2-succinylamino-6-oxoheptanedioateNot available
Average286.218Da
Monoisotopic286.0579371Da
BASm00004283-oxoadipateC6H6O5Chemical structure of 3-oxoadipateNot available
Average158.11Da
Monoisotopic158.022620453Da
BASm00004323,5-dichlorocatecholC6H4Cl2O2Chemical structure of 3,5-dichlorocatecholNot available
Average179.001Da
Monoisotopic177.9588348Da
BASm0000642S-adenosyl-4-methylsulfanyl-2-oxobutanoateC15H19N5O6SChemical structure of S-adenosyl-4-methylsulfanyl-2-oxobutanoateNot available
Average397.406Da
Monoisotopic397.105604055Da
BASm00007164-methylsulfanyl-2-oxobutanoateC5H7O3SChemical structure of 4-methylsulfanyl-2-oxobutanoateNot available
Average147.17Da
Monoisotopic147.012138839Da

Displaying 1–10 of 145 metabolites