Aminobacterium colombiense DSM 12261

Aminobacterium colombiense (strain DSM 12261 / ALA-1) is a strictly anaerobic, amino acid-fermenting, Gram-negative bacterium isolated from an anaerobic lagoon of a dairy wastewater treatment plant. This organism can grow on serine, threonine, glycine and pyruvate. Alanine, glutamate, valine, isoleucine, leucine, methionine, aspartate and malate are oxidized only in the presence of the hydrogenotroph, M. formicicum (H2 scavenger). In addition, the utilization of cysteine, threonine and alpha-ketoglutarate are also improved in the presence of M. formicicum. An 80% hydrogen atmosphere inhibits growth on threonine and alpha-ketoglutarate, whereas glycine degradation is not affected. Serine and pyruvate are partially affected by the presence of hydrogen. The fermentation of serine to acetate occurs via the conversion of serine to pyruvate and ammonia with a serine dehydratase. The optimal growth temperature is 37 degrees Celsius and the temperature range for growth is between 20 and 42 degrees Celsius, with no growth occurring at 18 and 45 degrees Celsius. It does not require NaCl for growth but tolerates less than 1.5% NaCl. Optimum growth occurs in the presence of 0.05 to 0.5%NaCl. The pH range for growth is 6.6 to 8.5 with an optimum pH around 7.3. (Adapted from PMID: 16887649). (EBI Integr8)