Methanohalophilus mahii DSM 5219

CocciNon-motileAnaerobe

Kingdom

Methanobacteriati

Phylum

Methanobacteriota

Class

Methanomicrobia

Order

Methanosarcinales

Family

Methanosarcinaceae

Genus

Methanohalophilus

Description

Methanohalophilus mahii (strain ATCC 35705 / DSM 5219 / SLP) is a coccoid, halophilic, methanogen archaeon isolated from sediment from the Great Salt Lake. Surface colonies are cream to pale yellow and circular and often appear foamy due to trapped gases. M. mahii is found in anaerobic sediments with salinity levels at or above seawater concentration. It requires sodium chloride concentrations in the moderately halophilic range of 1.0 to 2.5 M for optimal growth and methanogenesis. Trimethylamine, dimethylamine, methylamine, and methanol serve as substrates for growth. The optimum temperature, pH and salinity for growth and methanogenesis are 37 degrees Celsius, pH 7.5 and 2.0 M NaCl, respectively. (Adapted from : http://ijs.sgmjournals.org/cgi/reprint/38/1/122.pdf). (HAMAP: METMS)

Taxonomy

Kingdom	Methanobacteriati
Phylum	Methanobacteriota
Class	Methanomicrobia
Order	Methanosarcinales
Family	Methanosarcinaceae
Genus	Methanohalophilus
Species	Methanohalophilus mahii
Strain	DSM 5219

Profile

Physiology
Gram staining properties	Not Available
Shape	Cocci
Mobility	No
Flagellar presence	Yes
Number of membranes	1

Ecology, Host, and Life Cycle
Oxygen requirements	Anaerobe
Optimal temperature	Not Available
Temperature range	Mesophilic
Habitat	Specialized
Biotic relationship	Free living
Host(s)	Not Available
Cell arrangement	Not Available
Sporulation	Not Available
Energy source	Methylotroph
Pathogenicity	No

Genome Summary

Methanohalophilus mahii DSM 5219

Accession NumberNC_014002.1

Gene Summary

Adenine Count

572776 bp

Thymine Count

581989 bp

Guanine Count

428659 bp

Cytosine Count

429000 bp

Genome Length

2012424 bp

Protein-coding Genes

2023 genes

Non-Coding Genes

59 genes

# of Chromosomes/Plasmids

1

Genes

Name	Locus Tag	UniProt	Strand	Coordinates	Molecular Weight
malate dehydrogenase	MMAH_RS05535	Q8PVJ7	-	1130977 - 1131900	32854.9
gntp family permease	MMAH_RS05540	Not Available	+	1132097 - 1133332	44675.1
o-acetyl-adp-ribose deacetylase	MMAH_RS05545	Q8TQD0	+	1133373 - 1133894	18983.9
hypothetical protein	MMAH_RS05555	Not Available	-	1134272 - 1134679	15452.5
nyn domain-containing protein	MMAH_RS05560	Not Available	-	1134672 - 1135292	23661.6
hypothetical protein	MMAH_RS05565	Not Available	+	1135516 - 1136109	23191.1
hypothetical protein	MMAH_RS05570	Not Available	-	1136392 - 1137114	27802.4
hypothetical protein	MMAH_RS05575	Not Available	-	1137347 - 1137727	13757.5
hypothetical protein	MMAH_RS05580	Not Available	+	1137945 - 1138418	18248.8
metallophosphoesterase	MMAH_RS05585	Not Available	+	1139113 - 1139592	17242.5

Displaying genes 1141 – 1150 of 2082 in total

Pathways

0 pathways

No pathways found

No metabolic pathways have been associated with this bacterium yet.

Metabolite ID	Metabolite name	Structure	CAS number	Average mass Monoisotopic mass
BASm0000217	alpha-ribazoleC14H18N2O4		Not available	Average278.3037Da Monoisotopic278.126657074Da
BASm0000237	(R)-4'-phosphopantothenateC9H18NO8P		Not available	Average299.2149Da Monoisotopic299.0770031Da
BASm0000344	(2R,3S)-homoisocitrateC7H7O7		Not available	Average203.128Da Monoisotopic203.020823305Da
BASm0000377	(S)-malateC4H4O5		Not available	Average132.0716Da Monoisotopic132.005873238Da
BASm0000387	(6R)-5,10-methylene-5,6,7,8-tetrahydrofolateC20H21N7O6		Not available	Average455.432Da Monoisotopic455.1564286Da
BASm0000430	hercynineC9H15N3O2		Not available	Average197.238Da Monoisotopic197.1164267Da
BASm0000515	mycothioneC34H58N4O24S2		Not available	Average970.96Da Monoisotopic970.2882411Da
BASm0000908	propanoateC3H5O2		Not available	Average73.072Da Monoisotopic73.029502981Da
BASm0001035	indole-3-pyruvateC11H8NO3		`35656-49-6`	Average202.1861Da Monoisotopic202.0504181Da
BASm0001142	butanoateC4H7O2		Not available	Average87.099Da Monoisotopic87.045153045Da

Displaying 1–10 of 144 metabolites