Pseudomonas putida KT2440

Gram-negativeRodMotileAerobe

Kingdom

Pseudomonadati

Phylum

Pseudomonadota

Class

Gammaproteobacteria

Order

Pseudomonadales

Family

Pseudomonadaceae

Genus

Pseudomonas

Description

Pseudomonas putida KT2440 is a Gram-negative, rod-shaped bacterium that thrives in moderate temperatures, classified as a mesophile. This versatile microbe is a heterotroph, utilizing organic compounds as energy and carbon sources. P. putida inhabits various environments, including soil, water, and surfaces in both natural and engineered ecosystems, making it widely distributed across many ecological niches. As a Gram-negative organism, Pseudomonas putida possesses a thin peptidoglycan layer surrounded by an outer membrane, which contains lipopolysaccharides. This structural feature contributes to its robustness in diverse environments and enhances its ability to resist certain antibiotics. The rod-shaped morphology of Pseudomonas allows for motility through flagella, aiding in its adaptation and survival in fluctuating conditions. Pseudomonas putida KT2440 is classified as a facultative anaerobe, meaning it can adapt to both aerobic and anaerobic environments. This adaptability allows it to thrive in various ecological settings, including oxygen-rich environments, such as water and soil, as well as in low-oxygen conditions, such as sediments. The bacterium is well-known for its metabolic versatility; it can degrade a wide range of organic pollutants, including aromatic hydrocarbons and other xenobiotic compounds, making it a valuable organism in bioremediation efforts to clean up contaminated environments. Moreover, Pseudomonas putida KT2440 has been extensively studied for its potential in biotechnology applications. It possesses unique metabolic pathways that enable it to produce biodegradable plastics and biofuels, presenting avenues for sustainable development. The opportunistic nature of this microbe also makes it a fascinating subject of study in microbial ecology and synthetic biology, as researchers explore its capabilities for bioengineering and environmental restoration.

Taxonomy

KingdomPseudomonadati
PhylumPseudomonadota
ClassGammaproteobacteria
OrderPseudomonadales
FamilyPseudomonadaceae
GenusPseudomonas
SpeciesPseudomonas putida
StrainKT2440

Profile

Physiology
Gram staining propertiesNegative
ShapeRod
MobilityYes
Flagellar presenceYes
Number of membranes2
Image of Pseudomonas putida KT2440
Ecology, Host, and Life Cycle
Oxygen requirementsAerobe
Optimal temperatureNot Available
Temperature rangeMesophilic
HabitatMultiple
Biotic relationshipFree living
Host(s)Not Available
Cell arrangementSingles
SporulationNonsporulating
Energy sourceHeterotroph
PathogenicityNo

Genome Summary

Pseudomonas putida KT2440

Accession NumberAE015451

Gene Summary

Adenine Count

1186490 bp

Thymine Count

1192046 bp

Guanine Count

1913391 bp

Cytosine Count

1889946 bp

Genome Length

6181873 bp

Protein-coding Genes

5440048 genes

Non-Coding Genes

741825 genes

# of Chromosomes/Plasmids

2

Genes

NameLocus TagUniProtStrandCoordinatesMolecular Weight
AttlNot AvailableNot Available+1734867 - 1734885Not Available
Integrase family site-specific recombinasePP_RS07885P27077-1738086 - 173921942770.7
excisionasePP_RS07890Not Available-1739173 - 17394158988.65
hypothetical proteinPP_RS07895Not Available-1739449 - 173976611275.5
MethyltransferasePP_RS07900Not Available-1739823 - 174030518536.4
Hypothetical proteinPP_RS07905Not Available-1740302 - 174068513851.7
hypothetical proteinPP_RS07910Not Available-1740682 - 17409038087.44
hypothetical proteinPP_RS07915Not Available-1740906 - 17411639434.46
Hypothetical proteinPP_RS07920Not Available-1741160 - 174191228653.4
hypothetical proteinPP_RS07925Not Available-1741996 - 174286231987.1

Displaying genes 1 – 10 of 5703 in total

Pathways

10 pathways

Metabolites

386 records
Metabolite IDMetabolite nameStructureCAS number
BASm0000217alpha-ribazoleC14H18N2O4Chemical structure of alpha-ribazoleNot available
Average278.3037Da
Monoisotopic278.126657074Da
BASm0000232(4S)-perillyl alcoholC10H16OChemical structure of (4S)-perillyl alcoholNot available
Average152.237Da
Monoisotopic152.1201151Da
BASm0000237(R)-4'-phosphopantothenateC9H18NO8PChemical structure of (R)-4'-phosphopantothenateNot available
Average299.2149Da
Monoisotopic299.0770031Da
BASm00002502,5-didehydro-D-gluconateC6H7O7Chemical structure of 2,5-didehydro-D-gluconate53736-12-2
Average191.1156Da
Monoisotopic191.019177578Da
BASm00002512-dehydropantoateC6H9O4Chemical structure of 2-dehydropantoateNot available
Average145.1333Da
Monoisotopic145.050083776Da
BASm00002532-oxopent-4-enoateC5H5O3Chemical structure of 2-oxopent-4-enoateNot available
Average113.093Da
Monoisotopic113.024417601Da
BASm00002543-hydroxy-2-methylpropanoateC4H7O3Chemical structure of 3-hydroxy-2-methylpropanoateNot available
Average103.098Da
Monoisotopic103.0400677Da
BASm00002583-(carbamoylamino)propanoateC4H7N2O3Chemical structure of 3-(carbamoylamino)propanoateNot available
Average131.112Da
Monoisotopic131.046215673Da
BASm00002593alpha,12alpha-dihydroxy-7-oxo-5beta-cholanateC24H37O5Chemical structure of 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanateNot available
Average405.556Da
Monoisotopic405.264647871Da
BASm0000305tetrathionateO6S4Chemical structure of tetrathionateNot available
Average224.24Da
Monoisotopic223.8588696Da

Displaying 1–10 of 386 metabolites